• Actin assembly and endocytosis E.Y. Engqvist-Goldstein and David G. Drubin

    Actin Assembly and Endocytosis: From Yeast to Mammals | Annual ... Название: Actin assembly and endocytosis E.Y. Engqvist-Goldstein and David G. Drubin
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    Actin Assembly and Endocytosis: From Yeast to Mammals | Annual ...
    Åsa E.Y. Engqvist-Goldstein and David G. Drubin. Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202; email: ...

    Actin assembly and endocytosis E.Y. Engqvist-Goldstein and David G. Drubin

    Huntingtin-interacting protein 1 related (hip1r) is a novel component of clathrin-coated pits and vesicles and is a mammalian homologue of sla2p, an actin-binding protein important for both actin organization and endocytosis in yeast. First, real-time analysis of hip1ryfp and dsredclathrin light chain (lc) in live cells revealed that these proteins show almost identical temporal and spatial regulation at the cell cortex. .

    Second, at the ultrastructure level, immunogold labeling of unroofed cells showed that hip1r localizes to clathrin-coated pits. Here, we demonstrate that hip1r binds via its putative central coiled-coil domain to clathrin, and provide evidence that hip1r and clathrin are associated in vivo at sites of endocytosis. Consistent with a functional role for hip1r in endocytosis, we also demonstrated that it promotes clathrin cage assembly in vitro. In total, hip1rs properties suggest an early endocytic function at the interface between clathrin, f-actin, and lipids. Finally, we showed that hip1r is a rod-shaped apparent dimer with globular heads at either end, and that it can assemble clathrin-coated vesicles and f-actin into higher order structures.


    Actin Assembly and Endocytosis: From Yeast to ... - Annual Reviews


    ACTIN ASSEMBLY AND ENDOCYTOSIS: From Yeast to Mammals. ˚Asa E.Y. Engqvist-Goldstein and David G. Drubin. Department of Molecular and Cell ...

    Actin assembly and endocytosis E.Y. Engqvist-Goldstein and David G. Drubin

    RNAi-mediated Hip1R Silencing Results in Stable Association ...
    To learn more about the function of actin in clathrin-mediated endocytosis, ... levels of Hip1R provided evidence that F-actin polymerization occurs at coated- pits ..... just as actin's importance varies (reviewed in Engqvist-Goldstein and Drubin, ..... to cortactin (Engqvist-Goldstein, Å.E.Y., and D.G. Drubin, unpublished results).
    Actin assembly and endocytosis E.Y. Engqvist-Goldstein and David G. Drubin Keen, John Heuser, David G, Nov 13, 2003. Actin polymerization occurs late in the endocytic pathway and is required. Kessels, James H. ACTIN ASSEMBLY AND ENDOCYTOSIS: From Yeast to Mammals. Novel function of a dynein light chain in actin assembly during clathrin-mediated endocytosis. Drubin. to cortactin (Engqvist-Goldstein, Å. Drubin. Department of Molecular and Cell . Just as actin's importance varies (reviewed in Engqvist-Goldstein and Drubin, Engqvist-Goldstein. Engqvist-Goldstein and David G. E. Åsa E. Correspondence information about the author David G. Åsa E. Y. Huntingtin-interacting protein 1 related (hip1r) is a novel component of clathrin-coated pits and vesicles and is a mammalian homologue of sla2p, an actin-binding protein important for both actin organization and endocytosis in yeast. Drubin. E. Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202; email: .
  • The actin-binding protein Hip1R associates with clathrin during early ...


    . In total, hip1rs properties suggest an early endocytic function at the interface between clathrin, f-actin, and lipids. Second, at the ultrastructure level, immunogold labeling of unroofed cells showed that hip1r localizes to clathrin-coated pits. First, real-time analysis of hip1ryfp and dsredclathrin light chain (lc) in live cells revealed that these proteins show almost identical temporal and spatial regulation at the cell cortex. Here, we demonstrate that hip1r binds via its putative central coiled-coil domain to clathrin, and provide evidence that hip1r and clathrin are associated in vivo at sites of endocytosis. Consistent with a functional role for hip1r in endocytosis, we also demonstrated that it promotes clathrin cage assembly in vitro. Huntingtin-interacting protein 1 related (hip1r) is a novel component of clathrin-coated pits and vesicles and is a mammalian homologue of sla2p, an actin-binding protein important for both actin organization and endocytosis in yeast. Finally, we showed that hip1r is a rod-shaped apparent dimer with globular heads at either end, and that it can assemble clathrin-coated vesicles and f-actin into higher order structures.

    Sep 17, 2001 ... Åsa E.Y. Engqvist-Goldstein, Robin A. Warren, Michael M. Kessels, James H. Keen, John Heuser, David G. Drubin. Åsa E.Y. ... Novel function of a dynein light chain in actin assembly during clathrin-mediated endocytosis.

    A Pathway for Association of Receptors, Adaptors, and Actin during ...

    Nov 13, 2003 ... Correspondence information about the author David G. Drubin ..... Actin polymerization occurs late in the endocytic pathway and is required ...... to accumulate in association with endocytic proteins (Å.E.Y. Engqvist-Goldstein, ...
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  • Actin assembly and endocytosis E.Y. Engqvist-Goldstein and David G. Drubin

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